A prefoldin-associated WD-repeat protein (WDR92) is required for the correct architectural assembly of motile cilia

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A prefoldin-associated WD-repeat protein (WDR92) is required for the correct architectural assembly of motile cilia

WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To test this hypothesis, we performed an RNAi-based knockdown of WDR92 gene expr...

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Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

BACKGROUND Motile cilia on the inner lining of the oviductal epithelium play a central role in ovum transport toward the uterus and subsequent fertilization by sperm. While the basic ultrastructure of 9+2 motile cilia (nine peripheral microtubule doublets surrounding a central pair) has been characterized, many important steps of ciliogenesis remain poorly understood. RESULTS Our previous stu...

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The WD repeat-containing protein IFTA-1 is required for retrograde intraflagellar transport.

The assembly and maintenance of cilia require intraflagellar transport (IFT), a microtubule-dependent bidirectional motility of multisubunit protein complexes along ciliary axonemes. Defects in IFT and the functions of motile or sensory cilia are associated with numerous human ailments, including polycystic kidney disease and Bardet-Biedl syndrome. Here, we identify a novel Caenorhabditis elega...

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The Intraflagellar Transport Protein IFT20 Is Associated with the Golgi Complex and Is Required for Cilia Assembly□V

Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides that are thought to contain binding sites for various cargos that need to be transported from their site of synthesis in the cell body to the site of assembly in the cilium. We show here that the IFT...

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The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly.

Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides that are thought to contain binding sites for various cargos that need to be transported from their site of synthesis in the cell body to the site of assembly in the cilium. We show here that the IFT...

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ژورنال

عنوان ژورنال: Molecular Biology of the Cell

سال: 2016

ISSN: 1059-1524,1939-4586

DOI: 10.1091/mbc.e16-01-0040